Peptides are often treated like supplements you can stack for convenience. One for repair, one for metabolism, one for inflammation. That mindset leads people to assume they can simply mix peptides in the same syringe and inject once. The problem is peptides are not pills. They are fragile, information-carrying molecules whose behavior is governed by physics, chemistry, and biology at the same time. A peptide is not just a chain of amino acids. In solution it exists as a three-dimensional structure held together by weak forces like hydrogen bonds, electrostatic interactions, and hydrophobic effects. These forces are highly sensitive to the environment. Small changes in pH, ionic strength, or solvent conditions can change the peptide’s shape, stability, and behavior. When a peptide is manufactured, it is stabilized in a very specific formulation. That formulation controls pH, charge, ion balance, and solubility so the peptide stays folded correctly and remains biologically active. When you mix two peptides together, you destroy that controlled environment and create a new, untested chemical system. One of the first things that goes wrong is charge balance. Peptides carry electrical charge depending on pH. That charge helps keep molecules from sticking to each other. Mixing peptides can shift pH just enough to reduce repulsion between molecules. When repulsion drops, attraction wins, and peptides begin to stick together. Ionic strength matters too. Mixing solutions often increases ion concentration, which compresses the electrical “buffer” that keeps peptides apart. This allows molecules to drift close enough for hydrophobic regions to interact. Water dislikes exposed hydrophobic surfaces, so peptides clump together to lower free energy. This is basic solution physics. Once aggregation starts, it accelerates. A few misfolded molecules form a nucleus, which seeds further aggregation. Early clumps may be invisible, but they still matter. They reduce the amount of active peptide, alter absorption, and change signaling behavior.

In the latest DDT Method podcast with @Anthony Castore , Anthony discussed NAD supplementation. What I found interesting is that Anthony seemed to be against NAD supplementation via NAD+ and its precursors if I understood correctly, and a more appropriate approach would be to instead use a combination of 5 amino and 1MNA. He suggested using 5 amino pre workout and 1MNA on rest days in the evening. Anthony - would you be able to expand on your thoughts regarding NAD supplementation given it seems like a given in the longevity community that you supplement with NAD+ (injection or IV) or its precursors (NMN, NR).

Has anyone used any of the PEMF Devices that have Gauss powers in the 50-100 range? Most home mats (I have Healix) are 3 Gauss. Mine are 3 Gauss and frequency range is 1-50hz. I have read a lot on higher Gauss powers having the ability to penetrate deeper into tissues and the skeletal system. Right now this one seems to be the best bang for the buck: https://celler8.us/products/celler8 any insight/feedback/thoughts?

🚀 Ketones = Game Changer. This is the thread where I’ll drop my go-to ketone protocols ⚡️ and YOU can fire away with any questions. 💬 Comment your experiences, hacks, and questions below—let’s build the ultimate ketone resource together. 👀 Webinar coming soon… stay tuned. Practical Dosing Blueprints 1.Pre-Workout Protocol (Performance & Focus) Goal: Elevate ketones, buffer acidosis, support hydration & glucose supply. - Hydration: 500–750ml water + electrolytes (sodium 500–1000mg, potassium 200–400mg). - Bicarbonate: 0.3 g/kg sodium bicarbonate (~20g for 70kg athlete), dissolved in water. Take 90–120 min before session to minimize GI upset. - Trehalose (Carbohydrate): 15–25g as slow-release carb for sustained glycogen supply. - Ketone Ester (D-BHB/1,3-BD): 15–25ml (~7–12g KE) 10–15 min pre-warmup. Effect: Dual-fuel system (glucose + BHB), reduced acidosis, enhanced mental focus . 2.Intra-Workout Endurance Protocol Goal: Sustain metabolic efficiency, prevent bonk, extend time-to-exhaustion. - Every 60 min: Trehalose or isomaltulose: 20–30g (slow carb). Ketone Ester: 10–15ml (~5–7g KE). - Optional: electrolyte top-up (sodium/potassium). Effect: Preserves glycogen, stabilizes glucose, sustains BHB 1–3 mM . 3.Post-Workout Recovery Protocol Goal: Accelerate glycogen resynthesis, repair, and inflammation control. - Protein: 20–30g whey or EAAs. - Carbs: 40–60g high-GI glucose or maltodextrin. - Ketone Ester: 20–30ml (~10–15g KE), taken 30–45 min post-exercise (separate from carb/protein drink for maximal signaling). - Trehalose: Add 10–15g if training volume is very high or glycogen depleted. Effect: 50% faster glycogen replenishment, stronger mTOR activation, reduced inflammation . 4.Sleep Recovery Protocol (Athletes) Goal: Deep recovery, improve next-day performance. - Ketone Ester: 2.5–10ml (~1–5g KE) immediately before bed. - Optional: Magnesium glycinate/threonate for additional relaxation.

Whose used this? I have some, running 200mg a day, deffo sweat a bit more during cardio, if I combine it with SLU I get a noticeable increase in body temp. Not sure on dosing as see people saying once per day, multiple times etc. Anyone got experiences and share what they have found?