In Case You Missed The Study Session...
Greetings Gang, We had our live study session today! Here is the recording AND the summary of what we discussed! See you on the next one! VIEW RECORDING - 102 mins (No highlights) Meeting Purpose Review random LabCE questions for ASCP exam prep. Key Takeaways - Leukoreduction vs. Irradiation: Leukoreduction prevents CMV transmission (virus is in WBCs) and febrile reactions. Irradiation is required to prevent Graft-vs-Host Disease (GvHD) by inactivating all immunocompetent lymphocytes, a step leukoreduction alone cannot guarantee. - ABO Discrepancy Types: Group 2 discrepancies are caused by weak or missing antigens (e.g., A subgroups, acquired B phenomenon), while Group 1 is from weak or missing antibodies (e.g., newborns, elderly). - Hemolytic Uremic Syndrome (HUS): A key diagnosis for a patient with fever, elevated BP, low platelets, and schistocytes, especially with a gram-negative rod infection (often E. coli O157). - Spectrophotometry & Electrochemistry: Stray light is the primary cause of Beer's Law deviation. Electrochemistry is the basis for potentiometry, amperometry, and coulometry, which measure ions and gases (e.g., PO2 electrode). Topics Blood Bank & Transfusion - Leukoreduction vs. Irradiation - Leukoreduction: Filters WBCs from blood products.Prevents: CMV transmission (virus is in WBCs) and febrile reactions (recipient anti-WBC antibodies). Ineffective against: GvHD (some WBCs can pass filter), malaria (in RBCs), and bacteremia (bacteria are too small to be filtered). Irradiation: Inactivates all immunocompetent lymphocytes.Required for: Preventing GvHD in immunosuppressed patients. - ABO DiscrepanciesGroup 1 (Weak/Missing Antibodies) - Causes: Newborns, elderly, immunodeficiency. Result: Weak or negative reactions in the reverse grouping.Group 2 (Weak/Missing Antigens): Causes: A/B subgroups, leukemia, acquired B phenomenon. Result: Weak or negative reactions in the forward grouping. - Antibody Screen Interpretation - A positive screen requires the corresponding antigen to be present on the screening cells. If an antigen is absent, the antibody cannot be the cause of the reaction.