Hey y’all idk if this is the right place to ask this question but I am currently on the hunt for a job since I passed my exam earlier this year. I was interviewed but this one hospital via teams and I think the it went well. Long story short, they want me to come in and take a tour of the lab and also have a “peer interview” with their Tech Specialist and Technical Lead. Idk how to prep for this interview like should focus more on skill based questions they might ask me or what? I really want to get this job but idk if they want me since they said I have no actual lab experience except the rotations I did during school. Any help/tips will help. thx ☺️

My MLS exam is on the 21st of this month. I’ve been very blocked and i need to pass this time because it’s my second time applying. Because I don’t have much time left, i’m thinking of using the LSU purple and gold book, and the Valerie Polanski Cards. I’ve tried using the quick compendium book but i see a lot of pages and info and get overwhelmed. I also have LabCe that when i study a section and do questions on LabCe on it, I score good. Any recommendations or tips are welcomed…😭

Greetings Gang, We've had a few people pass their exams this week and one thing I noticed was that normal reference ranges weren't always been included next to CBC indices (or other questions involving lab results and disease state correlations). An excellent resource from the ASCP itself, is the ASCP Exam Content Outline. If you scroll down to about page 14, you'll find reference ranges for all indices tested on the exam. Here is the link to the newest version of the exam content outline ⬇️ https://ascpcontentwebsite.blob.core.windows.net/boccontent/docs/default-source/explore-credentials/content-guidelines/ascp_ascpi_mls_content_guideline.pdf?sfvrsn=3f02224_1 Attached are screenshots from the exam content outline. Hope this helps! - Marilyn

Hi everyone! Hope everyone is doing great and thriving in this MLT/MLS world of ours 🖤 I have a question, what helped yall throughout BloodBank and Microbiology classes? Any advice , things yall bought , etc will be greatly appreciated! It’s been hard me so anything will be great! Stay blessed 🖤❤️

Greetings Gang 😈🤪 HERE IS THE RECAP OF MONDAY'S LIVE STUDY SESSION ‼️✨There is another one tomorrow evening at 3PM PST/4PM MST/5PM CST/6PM EST https://fathom.video/share/DTxwmHy1sLVpYaFTAkb53canXdH-9NMC VIEW RECORDING - 88 mins (No highlights) Meeting Purpose Review ASCP practice questions across multiple lab disciplines. Key Takeaways - Lewis Antigens are Adsorbed: Lewis antigens are unique because they are not intrinsic to RBCs; they are passively adsorbed from plasma. This key distinction from other blood groups is a common exam point. - Yersinia enterocolitica is a Transfusion Risk: This organism's ability to grow at 4°C (blood storage temperature) makes it a critical cause of transfusion-related septicemia, a high-yield exam fact. - MDS Cytopenia is from Apoptosis: Myelodysplastic Syndromes (MDS) cause low cell counts (cytopenia) because the bone marrow produces abnormal cells that are destroyed via apoptosis before they can be released into circulation. - PCR Steps are Denaturation, Annealing, Extension (DAE): This is the correct sequence for PCR. "Hot start" methods prevent non-specific reactions by keeping the polymerase inactive until the optimal temperature is reached. Topics Molecular Biology & Genetics - DNA Nucleotide Composition:A DNA nucleotide consists of deoxyribose, phosphate, and a nitrogenous base (A, T, C, or G).Ribose and Uracil (U) are components of RNA only. - Huntington's Disease:Caused by a trinucleotide repeat: CAG.CGG is associated with Fragile X syndrome. - Second Messengers (cAMP vs. cGMP):cAMP is generated from ATP by adenyl cyclase.cGMP is generated from GTP by guanylyl cyclase.Clinical Relevance:cGMP → Blood vessel relaxation (vasodilation), explaining the action of drugs like nitroglycerin and .cAMP → Bronchodilation, explaining the action of drugs like albuterol.Lab Takeaway: High hormone levels with no expected effect can indicate a signal transduction problem (e.g., Type 2 Diabetes), not a hormone production issue. - PCR Steps & Hot Start PCR:Standard PCR Sequence: Denaturation → Annealing → Extension (DAE).Hot Start PCR: Prevents non-specific reactions by keeping the polymerase inactive until the optimal temperature is reached.Methods:Keeping the reaction on ice until the thermal cycler heats.Separating polymerase and primers with a wax bead that melts at high temperature.Using modified polymerases that are only activated at high temperatures. - MALDI-TOF Mass Spectrometry:Mechanism: Identifies bacteria by measuring the mass-to-charge ratio of molecules.Process: A laser vaporizes the sample, and the "time of flight" of the molecules is compared to a database for identification.Key Point: The laser does not amplify bacteria; it vaporizes them.