Thanks Cam, I really appreciate you taking the time to troubleshoot it. I'm actually still only using a search campaign, and i can't seem to find the edit ad group targeting button, it isn't appearing? In my audience manager i don't have a list with 1000+ people, does this mean i need to add a new audience from their in built audiences. Should i be turning off optimized targeting? It doesn't seem to let me remove any of the audiences in the similar segments audience/Your data segments tab? What do you advise i should do next? Thank you :)

@Cameron Walker Thanks Cameron, I'll do that now. Appreciate it.