How are we dosing glutathione?

Hey gang Wanted to ask advice So I have focused on a protocol to deal with my OID overactive immune disorder) I’ve got hashis and CIRS … lived in mold for years without knowing… now I’m 50 and in peri menopause. I had made some nice improvements but the last few months I hit a roadblock and things have gone sideways. Extreme exhaustion, air hunger, headaches, night sweats, dizziness and wooziness , rapid weight gain, increase in hunger that’s not normal… lots of eye and nose crunchies… I have been using TA1 for like 3 months… Amantadine, rapamycin… I take LDN… I realize many symptoms could be hormonal and peri menopause … I’ve used csm to treat the mild and toxins … I’ve got VIP and am waiting to use it until my recent Marcons re-test comes back … I want to use peptides to assist in repairing mitochondria..Ive researched a decent amount and have read frequently that there’s a peptide stack for peri : 2.5 mo protocol: • Reta - 0.5-1mg/wk • Mots-C. -5-10mg/wk • Bpc157 - 0.5-1mg daily • NAD+ - 15-50mg daily Curious thoughts and suggestions if anything has worked for anyone or your clients. Thanks for any input

The argument that mixed peptides are stable simply because a chromatography test showed high purity after 30 days does not hold up under basic principles of chemistry, molecular biology, or analytical science. The claim relies entirely on HPLC purity results, but HPLC only measures retention time and peak area. It does not prove that the molecular structure of a peptide is unchanged. A peptide can undergo oxidation, racemization, conformational changes, or aggregation and still appear as the same peak on a chromatogram. For example, oxidation of methionine to methionine sulfoxide changes the molecule chemically but often produces little or no shift in retention time. This means a sample can still appear 99% pure on HPLC even though part of the peptide population has been chemically altered. Detecting these types of structural changes requires more advanced techniques such as LC-MS/MS, peptide mapping, circular dichroism, NMR spectroscopy, capillary electrophoresis, or dynamic light scattering. None of those analyses were performed, so the conclusion that the peptides remained fully intact cannot be supported. Another major issue is the chemistry of copper and oxidation. When a copper-containing peptide such as GHK-Cu is mixed with other peptides, copper ions can catalyze oxidative reactions. Copper can participate in redox cycling that produces reactive oxygen species, which can oxidize amino acid side chains such as methionine, cysteine, tryptophan, tyrosine, and histidine. Methionine oxidation in particular is one of the most well-known stability problems in peptide drug formulation and pharmaceutical companies spend enormous resources preventing it. Even very small amounts of copper can catalyze these reactions, and the changes they produce may not be visible on a standard purity test. There is also the issue of peptide aggregation, which is governed by basic protein physics. Peptides in solution do not exist as isolated molecules. They constantly interact with water and with each other through hydrophobic interactions, electrostatic interactions, hydrogen bonding, and metal-mediated coordination. When multiple peptides are placed in the same solution, these interactions can create oligomers, aggregates, or misfolded complexes. Aggregation can dramatically change biological activity and receptor binding, yet aggregated peptides often still appear pure during chromatography testing because the test does not necessarily distinguish between properly folded and aggregated structures.

In the latest DDT Method podcast with @Anthony Castore , Anthony discussed NAD supplementation. What I found interesting is that Anthony seemed to be against NAD supplementation via NAD+ and its precursors if I understood correctly, and a more appropriate approach would be to instead use a combination of 5 amino and 1MNA. He suggested using 5 amino pre workout and 1MNA on rest days in the evening. Anthony - would you be able to expand on your thoughts regarding NAD supplementation given it seems like a given in the longevity community that you supplement with NAD+ (injection or IV) or its precursors (NMN, NR).

Anthony and friends - Dose anyone have any protocols in how to reduce/improve Lipoprotein(a)? - Dose any Peptides have risks factors? Thanks!