19h • General discussion
Stability Study of Semaglutide Over a 12-Month Period
Theorized hypothetical: You've given yourself your recently reconstituted GLP shot the Thursday before visiting your Great-Aunt Mable 2000 miles away for two weeks over the holidays. You inadvertently leave your GLP sitting out, unrefrigerated, on the counter, while you're gone. Your anxiety heightens once you realize you left it out. You think to yourself, "Well, that's money down the drain!"
Fear not! The study below researches stability and purity of Semaglutide (GLP1) after one year thanks to my vendor partner at Good Life Peptides. https://www.goodlifepeptides.com Code: KRISTINAMARIE
Abstract:
This study evaluated the stability of Semaglutide when stored under three different conditions—room
temperature (average 23 °C), refrigeration (average 4 °C), and freezing (average -17 °C)—over a 12-
month period. The Semaglutide used was obtained from Good Life Peptides
Parameters assessed included quantitative recovery and purity, determined using high-performance liquid chromatography (HPLC). The results demonstrated that Semaglutide maintained stability across all conditions, with minor variations in both quantity and purity.
Materials and Methods:
Sample Preparation-
Source: Semaglutide base vial (1.99 mg, 99.7% purity) purchased from Good Life Peptides.
Distribution: Nine vials were divided into three storage groups (n = 3 per condition):
o Room Temperature (average 23 °C)
o Refrigerator (average 4 °C)
o Freezer (average -17 °C)
Storage Equipment: Standard residential refrigerator and freezer unit.
Handling-
At the conclusion of the 12-month storage period, all vials were removed from their respective storage
conditions and brought to room temperature. Samples were then shipped under ambient conditions via
UPS 2-Day Air to Prime Analytical Labs for testing.
Analytical Testing:
Assay Method: High-performance liquid chromatography (HPLC)
Parameters Measured:
o Quantity (mg): Determined by HPLC quantification.
o Purity (%): Calculated as the percentage of intact Semaglutide from the chromatographic
profile.
Testing Points: Baseline (0 months) and after 12 months.
Results:
At 12 months, Semaglutide samples from each storage condition were analyzed. Results are summarized in Table 1.
Table 1. Stability of Semaglutide After 12 Months
Discussion:
Semaglutide demonstrated strong chemical stability under all three storage conditions over the course of 12 months. The quantity values showed slight increases compared to the baseline 1.99 mg, which may reflect variability in HPLC quantification or potential differences in sample concentration during
reconstitution.
Room Temperature (23 °C avg): Maintained high purity (99.17%), suggesting Semaglutide
remains chemically intact at ambient conditions. However, the slightly lower purity compared to
cold storage suggests some minimal degradation may occur over time.
Refrigerator (4 °C avg): Produced the highest recovery (2.30 mg) with strong purity (99.62%),
indicating refrigeration is a suitable and potentially optimal condition for long-term storage.
Freezer (-17 °C avg): Showed slightly lower recovery (2.15 mg) but the highest purity (99.76%),
suggesting freezing is effective for preserving structural integrity, though potential handling
factors during thawing could affect quantification.
It should be noted that the apparent increases in recovered quantity compared to the baseline 1.99 mg are unlikely to represent true “gain” of Semaglutide. Instead, these results most likely reflect manufacturer overfill of vials (common practice to ensure minimum label claim is met) combined with normal assay variability inherent to HPLC quantification.
Overall, Semaglutide retained excellent stability regardless of storage condition, with refrigerator and
freezer storage providing marginally better outcomes compared to room temperature.
Conclusion:
Semaglutide remains chemically stable for at least 12 months when stored at room temperature,
refrigerated, or frozen. Among these, refrigeration at 4 °C appears to provide the best balance of recovery and purity, while freezing preserves maximal purity. These findings support the use of standard
residential refrigeration or freezing for long-term storage of Semaglutide.
My two cents: This is why purity and quality should take priority over price.
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Stability Study of Semaglutide Over a 12-Month Period
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